InhaltsangabeSection One: TECHNOLOGIES Chapter One Energy Systems for ATP Regeneration in Cellfree Protein Synthesis Reactions Kara A. Calhoun, James R. Swartz Chapter 2 Continuousexchange Proteinsynthesizing Systems Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin Chapter 3 Cellfree production of integral membrane proteins on a preparative scale' Christian Klammt, Daniel Schwarz, Vilker Dötsch, Frank Bernhard Chapter 4 SIMPLEX: Singlemolecule PCRlinked in vitro expression: a novel method for highthroughput construction and screening of protein libraries Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano Chapter 5 Methods for Highthroughput Materialization of Genetic Information Based on Wheat Germ Cellfree Expression System Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo Chapter 6 Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti Section Two: APPLICATIONS Chapter 7 In vitro translation to study HIV protease activity Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi Chapter 8 The protein truncation test in mutation detection and molecular diagnosis Oliver Hauss, Oliver Müller Chapter 9 Creation of Novel Enantioselective lipases by SIMPLEX Yuichi Koga, Tsuneo Yamane, Hideo Nakano Chapter 10 In vitro transcription and translation coupled to 2Delectrophoresis for bacterial proteome analysis Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi Chapter 11 In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis Orit Gat, Haim Grosfeld,Avigdor Shafferman Chapter 12 Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli Chapter 13 Production of Protein for NMR Study Using the Wheat Germ CellFree System Toshiyuki Kohno, Yaeta Endo

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